Molecular characterization and phylogenetic analysis of feline hemoplasmas in domestic cats in Iran

Authors

  • Fereshteh Ghazisaeedi Department of Veterinary Internal Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Iraj Ashrafi Tamai Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Nahid Atyabi Department of Veterinary Internal Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Saeid Tabatabaei Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Solmaz Chegeni Department of Veterinary Internal Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Taghi Zahraei Salehi Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Abstract:

Three known feline hemoplasmas are Mycoplsama haemofelis, ‘Candidatus Mycoplasma haemominutum’ and ‘Candidatus Mycoplasma turicensis’. They are described as cause of feline infectious anemia in domestic and wild felids. Other blood parasites or blood-related pathogens like concurrent retroviral infections may deteriorate the clinical condition and severity of anemia. The aims of this study were molecular characterization and phylogenetic analysis of hemoplasmas in domestic cats in Iran for the first time. Blood samples were collected from 185 healthy and diseased domestic cats. Blood smears were prepared and hematological parameters were measured to determine possible anemia. Using 16S rRNA gene universal and species specific polymerase chain reactions with the following sequencing, 47 (25.40%) of cats were hemoplasma positive. Also, 17.02%, 72.50% and 40.40% of total positive samples were M. haemofelis, ‘Ca. M. haemominutum’ and ‘Ca.M. turicensis’ infected, respectively. 10 (21.20%) of hemoplasma positive cats had anemic blood profiles (HCT < 24.00%). All M. haemofelis infected cases were included. Partial 16S rRNA gene phylogenetic analysis revealed a high identity between the hemoplasma species found in this study and domestic cat sequences existing in GenBank. Phylogenetic analysis revealed 94.00% to 100% sequence identity between sequences of this study and existing sequences in Genbank. All hemoplasma isolates in this study were grouped within a single clade and additionally subdivided into two groups; haemofelis group including M. haemofelis and ‘Ca. M. turicensis’ and haemominutum group including ‘Ca. M. haemominutum’.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

molecular characterization and phylogenetic analysis of feline hemoplasmas in domestic cats in iran

three known feline hemoplasmas are mycoplsama haemofelis, ‘candidatus mycoplasma haemominutum’ and ‘candidatus mycoplasma turicensis’. they are described as cause of feline infectious anemia in domestic and wild felids. other blood parasites or blood-related pathogens like concurrent retroviral infections may deteriorate the clinical condition and severity of anemia. the aims of this study were...

full text

Molecular detection of feline hemoplasmas in feral cats in Korea.

The purpose of this study was to determine if Mycoplasma haemofelis, 'Candidatus Mycoplasma haemominutum' exist in Korea. Three hundreds and thirty one feral cats were evaluated by using PCR assay targeting 16S rRNA gene sequence. Fourteen cats (4.2%) were positive for M. haemofelis, 34 cats (10.3%) were positive for 'Candidatus M. haemominutum' and 18 cats (5.4%) were positive for both species...

full text

molecular characterization and phylogenetic analysis of feline hemotropic mycoplasmas

background: feline hemotropic mycoplasmas are parasites of erythrocytes and include three species, mycoplasma haemofelis, candidatus mycoplasma haemominutum and candidatus mycoplasma turicensis. diagnosis of the infection with these microorganisms can be carried out using conventional assays such as blood cytology. however, these assays have a low accuracy and a high rate of false-positive resu...

full text

Phylogenetic analysis of feline immunodeficiency virus in feral and companion domestic cats of New Zealand.

Nested PCR was used to amplify envelope V3-V6 gene fragments of feline immunodeficiency virus (FIV) from New Zealand cats. Phylogenetic analyses established that subtypes A and C predominate among New Zealand cats, with clear evidence of intersubtype recombination. In addition, 17 sequences were identified that were distinct from all known FIV clades, and we tentatively suggest these belong to ...

full text

role of cultural iran in promotion of multilaieralism in central asia and caucasus

cultural iran is a scope that is more extended than the political territories of iran as a political unit. this concept means that cultural geography(mehdi moghanlo-1383-1) of iran is greater than its political geography which, according to history, has a long history extending west-east from kandahar to the euphrates and north-south from the persian gulf to the caucasus including transoxiana a...

15 صفحه اول

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 8  issue 1

pages  67- 73

publication date 2017-03-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023